0.5mm rat heart matrix slicer Search Results


theophylline  (Thermo Fisher)
95
Thermo Fisher theophylline
FIGURE 3 | Ectopic expression of DdbA. (A) Cos-7 cells were infected with Ctr transformed with <t>theophylline</t> inducible expression plasmids controlling the production of HctA, DdbA, GFP (clover) or Scc1. Infected cells were induced for expression at 14 hpi and infectious progeny were isolated at 30 hpi. Progeny were quantified using an inclusion forming reinfection assay. Both HctA and DdbA induction resulted in a significant reduction in EB production (p < 0.01). While induction of Scc1 and GFP had no significant effect on EB production. (B) The morphological effects of ectopic expression was assessed by confocal microscopy. Cos-7 cells were infected with transformed Ctr expressing HctA, DdbA and Scc1, induced at 16 hpi, fixed at 24 hpi and stained with an anti-flag antibody to localize the expressed proteins. Additionally DNA morphology was assessed using DAPI staining. HctA expression resulted in a distinct change in the DAPI staining morphology, causing densely compacted regions of DNA in the RB. DdbA expression caused an increase in RB size but did not appreciatively change the compaction of the DNA. Scc1 expression had no effect on RB or DNA morphology. Size bar = 5µm. *p < 0.01.
Theophylline, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/0%2E5mm+rat+heart+matrix+slicer/Theophylline/pm34368013-83-18-19
Average 95 stars, based on 1 article reviews
theophylline - by Bioz Stars, 2026-11
95/100 stars
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pbs mc  (Thermo Fisher)
99
Thermo Fisher pbs mc
FIGURE 3 | Ectopic expression of DdbA. (A) Cos-7 cells were infected with Ctr transformed with <t>theophylline</t> inducible expression plasmids controlling the production of HctA, DdbA, GFP (clover) or Scc1. Infected cells were induced for expression at 14 hpi and infectious progeny were isolated at 30 hpi. Progeny were quantified using an inclusion forming reinfection assay. Both HctA and DdbA induction resulted in a significant reduction in EB production (p < 0.01). While induction of Scc1 and GFP had no significant effect on EB production. (B) The morphological effects of ectopic expression was assessed by confocal microscopy. Cos-7 cells were infected with transformed Ctr expressing HctA, DdbA and Scc1, induced at 16 hpi, fixed at 24 hpi and stained with an anti-flag antibody to localize the expressed proteins. Additionally DNA morphology was assessed using DAPI staining. HctA expression resulted in a distinct change in the DAPI staining morphology, causing densely compacted regions of DNA in the RB. DdbA expression caused an increase in RB size but did not appreciatively change the compaction of the DNA. Scc1 expression had no effect on RB or DNA morphology. Size bar = 5µm. *p < 0.01.
Pbs Mc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/0%2E5mm+rat+heart+matrix+slicer/Phosphate/keil_jeffrey_mccartney__2011__isolation_and_characterization_of_neuronal_substrates_of_the_ubiquitin_proteasome_system-614-14-40
Average 99 stars, based on 1 article reviews
pbs mc - by Bioz Stars, 2026-11
99/100 stars
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stainless steel brain matrix  (Stoelting inc)
95
Stoelting inc stainless steel brain matrix
FIGURE 3 | Ectopic expression of DdbA. (A) Cos-7 cells were infected with Ctr transformed with <t>theophylline</t> inducible expression plasmids controlling the production of HctA, DdbA, GFP (clover) or Scc1. Infected cells were induced for expression at 14 hpi and infectious progeny were isolated at 30 hpi. Progeny were quantified using an inclusion forming reinfection assay. Both HctA and DdbA induction resulted in a significant reduction in EB production (p < 0.01). While induction of Scc1 and GFP had no significant effect on EB production. (B) The morphological effects of ectopic expression was assessed by confocal microscopy. Cos-7 cells were infected with transformed Ctr expressing HctA, DdbA and Scc1, induced at 16 hpi, fixed at 24 hpi and stained with an anti-flag antibody to localize the expressed proteins. Additionally DNA morphology was assessed using DAPI staining. HctA expression resulted in a distinct change in the DAPI staining morphology, causing densely compacted regions of DNA in the RB. DdbA expression caused an increase in RB size but did not appreciatively change the compaction of the DNA. Scc1 expression had no effect on RB or DNA morphology. Size bar = 5µm. *p < 0.01.
Stainless Steel Brain Matrix, supplied by Stoelting inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/0%2E5mm+rat+heart+matrix+slicer/Steel+Brain+Matrix/pmc12400197-131-8-15
Average 95 stars, based on 1 article reviews
stainless steel brain matrix - by Bioz Stars, 2026-11
95/100 stars
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rat heart slicer  (Zivic Laboratories Inc)
86
Zivic Laboratories Inc rat heart slicer
FIGURE 3 | Ectopic expression of DdbA. (A) Cos-7 cells were infected with Ctr transformed with <t>theophylline</t> inducible expression plasmids controlling the production of HctA, DdbA, GFP (clover) or Scc1. Infected cells were induced for expression at 14 hpi and infectious progeny were isolated at 30 hpi. Progeny were quantified using an inclusion forming reinfection assay. Both HctA and DdbA induction resulted in a significant reduction in EB production (p < 0.01). While induction of Scc1 and GFP had no significant effect on EB production. (B) The morphological effects of ectopic expression was assessed by confocal microscopy. Cos-7 cells were infected with transformed Ctr expressing HctA, DdbA and Scc1, induced at 16 hpi, fixed at 24 hpi and stained with an anti-flag antibody to localize the expressed proteins. Additionally DNA morphology was assessed using DAPI staining. HctA expression resulted in a distinct change in the DAPI staining morphology, causing densely compacted regions of DNA in the RB. DdbA expression caused an increase in RB size but did not appreciatively change the compaction of the DNA. Scc1 expression had no effect on RB or DNA morphology. Size bar = 5µm. *p < 0.01.
Rat Heart Slicer, supplied by Zivic Laboratories Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/0%2E5mm+rat+heart+matrix+slicer/adult+brain+matrix+mouse+slicer/bio_rxiv__64898__2026__05__01__722023-58-22-25
Average 86 stars, based on 1 article reviews
rat heart slicer - by Bioz Stars, 2026-11
86/100 stars
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insyte-wtm intravenous bladder catheter  (Becton Dickinson)
90
Becton Dickinson insyte-wtm intravenous bladder catheter
FIGURE 3 | Ectopic expression of DdbA. (A) Cos-7 cells were infected with Ctr transformed with <t>theophylline</t> inducible expression plasmids controlling the production of HctA, DdbA, GFP (clover) or Scc1. Infected cells were induced for expression at 14 hpi and infectious progeny were isolated at 30 hpi. Progeny were quantified using an inclusion forming reinfection assay. Both HctA and DdbA induction resulted in a significant reduction in EB production (p < 0.01). While induction of Scc1 and GFP had no significant effect on EB production. (B) The morphological effects of ectopic expression was assessed by confocal microscopy. Cos-7 cells were infected with transformed Ctr expressing HctA, DdbA and Scc1, induced at 16 hpi, fixed at 24 hpi and stained with an anti-flag antibody to localize the expressed proteins. Additionally DNA morphology was assessed using DAPI staining. HctA expression resulted in a distinct change in the DAPI staining morphology, causing densely compacted regions of DNA in the RB. DdbA expression caused an increase in RB size but did not appreciatively change the compaction of the DNA. Scc1 expression had no effect on RB or DNA morphology. Size bar = 5µm. *p < 0.01.
Insyte Wtm Intravenous Bladder Catheter, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/0%2E5mm+rat+heart+matrix+slicer/catheter+bd+insyte/pm27260964-75-2-1
Average 90 stars, based on 1 article reviews
insyte-wtm intravenous bladder catheter - by Bioz Stars, 2026-11
90/100 stars
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rat bmscs adipogenic differentiation medium  (Procell Inc)
86
Procell Inc rat bmscs adipogenic differentiation medium
FIGURE 3 | Ectopic expression of DdbA. (A) Cos-7 cells were infected with Ctr transformed with <t>theophylline</t> inducible expression plasmids controlling the production of HctA, DdbA, GFP (clover) or Scc1. Infected cells were induced for expression at 14 hpi and infectious progeny were isolated at 30 hpi. Progeny were quantified using an inclusion forming reinfection assay. Both HctA and DdbA induction resulted in a significant reduction in EB production (p < 0.01). While induction of Scc1 and GFP had no significant effect on EB production. (B) The morphological effects of ectopic expression was assessed by confocal microscopy. Cos-7 cells were infected with transformed Ctr expressing HctA, DdbA and Scc1, induced at 16 hpi, fixed at 24 hpi and stained with an anti-flag antibody to localize the expressed proteins. Additionally DNA morphology was assessed using DAPI staining. HctA expression resulted in a distinct change in the DAPI staining morphology, causing densely compacted regions of DNA in the RB. DdbA expression caused an increase in RB size but did not appreciatively change the compaction of the DNA. Scc1 expression had no effect on RB or DNA morphology. Size bar = 5µm. *p < 0.01.
Rat Bmscs Adipogenic Differentiation Medium, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/0%2E5mm+rat+heart+matrix+slicer/chondrogenic+medium/pm41969062-314-9-15
Average 86 stars, based on 1 article reviews
rat bmscs adipogenic differentiation medium - by Bioz Stars, 2026-11
86/100 stars
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sodium carbonate/oxalic acid concentrate  (Aladdin Scientific Corporation)
N/A
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Image Search Results


FIGURE 3 | Ectopic expression of DdbA. (A) Cos-7 cells were infected with Ctr transformed with theophylline inducible expression plasmids controlling the production of HctA, DdbA, GFP (clover) or Scc1. Infected cells were induced for expression at 14 hpi and infectious progeny were isolated at 30 hpi. Progeny were quantified using an inclusion forming reinfection assay. Both HctA and DdbA induction resulted in a significant reduction in EB production (p < 0.01). While induction of Scc1 and GFP had no significant effect on EB production. (B) The morphological effects of ectopic expression was assessed by confocal microscopy. Cos-7 cells were infected with transformed Ctr expressing HctA, DdbA and Scc1, induced at 16 hpi, fixed at 24 hpi and stained with an anti-flag antibody to localize the expressed proteins. Additionally DNA morphology was assessed using DAPI staining. HctA expression resulted in a distinct change in the DAPI staining morphology, causing densely compacted regions of DNA in the RB. DdbA expression caused an increase in RB size but did not appreciatively change the compaction of the DNA. Scc1 expression had no effect on RB or DNA morphology. Size bar = 5µm. *p < 0.01.

Journal: Frontiers in cellular and infection microbiology

Article Title: The sRNA Regulated Protein DdbA Is Involved in Development and Maintenance of the Chlamydia trachomatis EB Cell Form.

doi: 10.3389/fcimb.2021.692224

Figure Lengend Snippet: FIGURE 3 | Ectopic expression of DdbA. (A) Cos-7 cells were infected with Ctr transformed with theophylline inducible expression plasmids controlling the production of HctA, DdbA, GFP (clover) or Scc1. Infected cells were induced for expression at 14 hpi and infectious progeny were isolated at 30 hpi. Progeny were quantified using an inclusion forming reinfection assay. Both HctA and DdbA induction resulted in a significant reduction in EB production (p < 0.01). While induction of Scc1 and GFP had no significant effect on EB production. (B) The morphological effects of ectopic expression was assessed by confocal microscopy. Cos-7 cells were infected with transformed Ctr expressing HctA, DdbA and Scc1, induced at 16 hpi, fixed at 24 hpi and stained with an anti-flag antibody to localize the expressed proteins. Additionally DNA morphology was assessed using DAPI staining. HctA expression resulted in a distinct change in the DAPI staining morphology, causing densely compacted regions of DNA in the RB. DdbA expression caused an increase in RB size but did not appreciatively change the compaction of the DNA. Scc1 expression had no effect on RB or DNA morphology. Size bar = 5µm. *p < 0.01.

Article Snippet: Cos7 cells on coverslips were infected with Ctr-E-DdbA3xFlag, CtrE-HctA3xFlag, Ctr-E-Scc13xFlag strains and induced at 16 hpi with 0.5mM theophylline (Acros Organics, Thermo ScientificTM).

Techniques: Expressing, Infection, Transformation Assay, Isolation, Confocal Microscopy, Staining